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. 2019 Mar 22;14(3):e0213598. doi: 10.1371/journal.pone.0213598

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© 2019 Spector et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — A, RNA generated during a 3-minute limiting UC pulse with or without a subsequent chase on a template with a 503 nt runoff. Excess P-TEFb (P) or flavopiridol (F) were added during PIC formation. Indicated complexes were chased (C) for 3 minutes in the absence or presence of flavopiridol (FC). B, EC-EMSA of EECs generated in the presence of flavopiridol or P-TEFb and pulsed with limiting UC for 3 minutes. Complexes were stopped with EDTA and incubated for the indicated times prior to HSW (1.6 M KCL) and LSW (60 mM KCl). C, quantification of phosphatase activity in B. Total amount of counts within the unphosphorylated band (UnP) was divided be total counts within the lane to determine percent phosphatased.