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. 2018 Nov 6;52(1):e12524. doi: 10.1111/cpr.12524

Figure 4.

Figure 4

Lipid production by sebocytes and glutamine effect revealed by flow cytometry. A, Neutral lipid production by sebocytes grown 3 weeks with (Seb4Gln) or without glutamine (Seb4) revealed by FL1 channel after Nile Red staining. Green and Seb5 media were tested as control. B, Nile Red MFI obtained with the four different channels FL1, FL2, FL3 and FL4 in the same conditions. C, Dot plots showing Nile Red signal in FL1 channel (neutral lipids—C1, C2, C4, C5) or FL4 channel (polar lipids—C3, C6) for sebocytes grown 7 days with (Seb4Gln—C5, C6) or without glutamine (Seb4—C2, C3). Sebocytes grown in Seb5 (C1) and Green medium (C4) were used as control. NR‐dim and NR‐bright, sebocyte population with, respectively, low and high Nile Red signal. D, Nile Red MFI ratio between NR‐bright and NR‐dim populations after 7 days (Week1) or 21 days (Week3) of sebocyte culture in Seb4 and Seb4Gln media. One repetition was done each week