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The results of Alkaline phosphatase staining and alizarin red staining. The colour of EMSCs in ALP assay was gradually deeper with the increase in development days and becomes the deepest at E18.5 d after a 7‐day mineralized induction. The alizarin red staining also showed that there were more and bigger mineralized nodules in the E18.5 d and E15.5 d EMSCs than that in the E13.5 d and E12.5 d EMSCs after a 21‐day mineralized induction. Scale bar represents 50 μm
