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. 2018 Oct 19;52(1):e12514. doi: 10.1111/cpr.12514

Figure 4.

Figure 4

Knockdown of SREBP1 suppresses the stemness and resveratrol rescues the stemness induced by gemcitabine via suppressing SREBP1. (A) Sphere formation assays were performed after knockdown of SREBP1. The images were taken at a magnification of 200× on the 7th day. Scale bars = 50 μm. The numbers of sphere were counted. **P < 0.01 compared with the control group. (B) The Sox2, Oct4, and Nanog expression levels after knockdown of SREBP1 were detected by western blotting. *P < 0.05, **P < 0.01, or ***P < 0.001 compared with the control group. (C) After resveratrol (50 μmol/L) and gemcitabine (5 μmol/L) treatment for 48 h, pancreatic cancer (PC) cells were subjected to the tumour sphere‐forming assay. The sphere numbers of the MiaPaCa‐2 and Panc‐1 cells were counted. The magnification of the graph is 200×. Scale bars = 50 μm. (D) The effects of resveratrol (50 μmol/L) and gemcitabine (5 μmol/L) treatment on the Sox2, Oct4, and Nanog expression levels were detected by western blotting. (E) After treatment with SiSREBP1 combined with gemcitabine (5 μmol/L), western blotting was performed to detect the Sox2, Oct4, and Nanog expression levels in the MiaPaCa‐2 and Panc‐1 cell lines. ns, not significant, *P < 0.05, **P < 0.01, or ***P < 0.001