Table 1.
The differentiation media for mouse female germline stem cells
| Medium 1 | Medium 2 | Medium 3 | Medium 4 | Medium 5a | Medium 5b | Medium 5c | |
|---|---|---|---|---|---|---|---|
| FBS (%) | 10 | 10 | 10 | 10 | 10 | 10 | 15 |
| NEAA (m mol L−1) | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| L‐Glu (m mol L−1) | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| Sodium pyruvate (m mol L−1) | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| β‐mercaptoethanol (m mol L−1) | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Penicillin (μg/mL) | 15 | 15 | 15 | 15 | 15 | 15 | 15 |
| LIF | — | — | — | — | — | — | — |
| bFGF (ng/mL) | 20 | 20 | 20 | 20 | — | 10 | 10 |
| EGF (ng/mL) | 10 | 10 | 10 | — | — | — | 10 |
| GDNF (ng/mL) | 10 | 10 | 10 | — | — | — | — |
| BMP4 (ng/mL) | — | — | — | — | — | 10 | — |
| RA (M) | 10−7 | — | 10−7 | — | — | 10−7 | — |
| Ovarian homogenate (μL) | — | 100 | 100 | — | — | — | 100 |
| Oestrogen (ng/mL) | — | — | — | 1 | — | — | 1 |
| Progesterone (ng/mL) | — | — | — | 1 | — | — | 1 |
| PMSG (IU/mL) | — | — | — | — | — | — | 1 |
| hCG (IU/mL) | — | — | — | — | — | — | 1 |
| Transferrin (μg/mL) | — | — | — | — | — | — | 5 |
| Insulin (μg/mL) | — | — | — | — | — | — | 10 |
| MEM‐α | Up to 10 mL | Up to 10 mL | Up to 10 mL | Up to 10 mL | Up to 10 mL | Up to 10 mL | Up to 10 mL |
| Granulosa cells | — | — | — | — | — | + | + |
| Hanging drop | — | — | — | — | + | — | — |