Figure 3.

Changes in bone turnover marker levels at 12 weeks vs baseline in patients with or without bone metastases at baseline. The percentage change in bone turnover marker levels was calculated from the difference at 12 weeks vs baseline for patients with or without bone metastases at baseline. Blood samples (8.5mL, fasting preferred) were collected at baseline and at 6 and 12 weeks (visits 5 and 6) after treatment initiation. Serum bone-specific alkaline phosphatase (BSAP) was measured with an immunochemiluminescence assay using the Ostase reagent on an automatic analyzer. Serum amino-terminal propeptide of type 1 collagen (P1NP) was measured with a two-site immunoassay using monoclonal antibodies against purified human P1NP to detect both intact monomeric and trimeric forms, but not fragments. Serum C-terminal cross-linking telopeptide of type 1 collagen (CTX) was measured with a two-site assay using monoclonal antibodies against an eight amino acid sequence from the C-telopeptide of human type 1 collagen. Error bars are standard error of the mean. P values are two-sided.