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. 2018 Aug 21;13(9):2427–2432. doi: 10.1021/acschembio.8b00639

Figure 1.

Figure 1

Impact of (+) and (−) side nucleotides on substrate turnover. (A) Representative 1H NMR spectra recorded over a time course are shown for the 10-mer 5′-TTATTCATAT-3′, dCMP and 5′-TCA-3′. The intensities of the C peak were used to monitor substrate turnover and to calculate the initial reaction rates. (B) Time course of deamination rates for 10-mer 5′-TTATTCATAT-3′, dCMP, and (+) and (−) side only oligonucleotides. (C) Addition of +1A (left) and two T nucleotides at the (−) side (right) increase turnover of the substrate oligo, whereas a single T on the (−) side appears detrimental. ns not significant, *p-value < 0.05, ***p-value < 0.001, One-way ANOVA and Bonferroni test.