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. 2019 Mar 22;14(3):e0214254. doi: 10.1371/journal.pone.0214254

Fig 3. Insulin signalling activation in skeletal muscle biopsies.

Fig 3

(A) Representative western blot analysis of the expression and phosphorylation of proteins involved in the insulin pathway. Skeletal muscle samples were incubated in absence (-) or presence (+) of 10 nM insulin for 20 minutes. Due to the high number of samples analysed, the figure panel is composed by images of two different gels. Details on how western blot experiments were performed are reported in Materials and Methods. (B) Fold change on basal level of the quantification of IRS1, AS160, AKT/PKB, p70S6K and ERK1/2 activation. Histograms represent mean values and bars represent standard error of the mean (SEM). The number of samples analysed in each group is reported in graphic legend. Differences between groups have been evaluated by Student t-test. *p<0.05; **p<0.01; ****p<0.0001.