FIG 10.

Comparison of serum neutralization potency across vaccination regimens. The neutralization potency (NP) of each serum sample collected at week 40/41 (A) and week 58 (B) was calculated using the neutralization index tool from the Los Alamos HIV Database and is plotted on the y axis. The tool considers the neutralization susceptibility of each of the 12 reference Envs, as shown in Fig. 9. Each symbol represents the NP score of an individual monkey serum sample. An NP score of ∼1 indicates that a serum sample can be expected to neutralize only tier 1 Envs, while serum with increasing NP scores can be expected to neutralize more resistant tier 2 and 3 Envs. Most of the serum samples in this analysis had NP scores of 0.92 or 1.92, with a subset having NP scores exceeding 2. A Kruskal-Wallis test was used to determine whether the NP scores were significantly different from those found in the DDMMM-immunized animals. The DDMMPP immunization group was significantly different from the DDMMM group at both week 40 and week 58; the protein-only group was significantly different from the DDMMM group only at week 58 (*, P < 0.05; **, P < 0.01; ****, P < 0.0001). The adjuvant used with the protein immunizations is indicated as Adjuplex (ADJ) or 3M-052.