FIG 8.

Serum neutralization of the global HIV-1 Env reference panel. Heatmaps were generated to portray the neutralizing capacity of heat-inactivated immunized monkey serum from the peak response at week 40 for M19 and M22 and week 41 for M21 (A) and on the day of challenge at week 58 (B). A pool of naive monkey serum was included as a negative control against each Env (the last row in each heat map). Each heat-inactivated serum sample was diluted 1:50 and analyzed in duplicate against the HIV-1 Env pseudoviruses indicated at the top of each heat map using the TZM-bl cell assay. The Envs included the global reference panel, listed from the most to the least sensitive, as described previously (23) (n = 12); a representative Env cloned from the SHIV1157ipd3N4 challenge stock; and the MLV Env as a negative control. The trials are indicated at the top of the figure. For each heat map, the Envs are indicated at the top, and the vaccination arms are indicated to the left. The key presents the percent residual infectivity of each Env pseudovirus in the presence of each serum sample, represented as color. The color scale is the same for each heat map and indicates the potency of neutralization: red, good potency (1 to 25% residual infectivity); blue, fair potency (26 to 50% residual infectivity); green, weak potency (51 to 75% residual infectivity); and black, poor potency (greater than 76% residual infectivity). White boxes with an X, a serum-Env combination was not analyzed due to limited availability, necropsy, or poor sample quality. Vec, vector.