FIG 5.

PR mutations decrease gp120 association and increase gp120 shedding. (A) Representative results of gp120 shedding of ³⁵S-labeled WT and mutant Env (M1 to M5) from one of four separate experiments. HEK293T cells were transfected with WT or mutant Env expression constructs for the gp120 shedding assay. The gp120 processing and the association index were calculated as described in Materials and Methods and are shown below the shedding gel. Values are representative of the average of four separate experiments. (B) Summary results of four independent experiments of gp120 shedding. Dunnett's multiple-comparison test was used for statistical analysis. ***, P < 0.001, for a comparison of the association index of mutant M1, M3, or M4 with that of WT Env.