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PCR primers used in generating HIV-1 gp41 mutants M1 to M6
| PCR primer | DNA sequence (5′–3′)a |
|---|---|
| M1 | TGGGCGCAGCGCCAATGGCGCTGACGGTACA |
| M2 | TGGGCGCAGCGTCAATGACGCTGGCGGTACA |
| M3 | TGGGCGCAGCGCCAATGGCGCTGGCGGTACA |
| M4 | TGGGCGCAGCGCCAATGACGCTGACGGTACA |
| M5 | TGGGCGCAGCGTCAATGGCGCTGACGGTACA |
| M6 | TGGGCGCAGCGGCAATGACGCTGACGGTACA |
Mutated nucleotides compared with HIVNL4-3 sequence (GenBank accession number M19921.2) are shown in boldface.
