FIG 2.

Expression of PRV gB alone, in the absence of other viral proteins, is sufficient to trigger NK cell-mediated lysis of target cells. (A) RK13 and RN/008 cells were incubated with IL-2-primed porcine primary NK cells at target/effector cell ratios of 1:12, 1:6, and 1:3 for 4 h at 37°C. Viability of target cells was assessed by propidium iodide staining and flow cytometric analysis, and the percent NK cell-mediated lysis was calculated. Data represent mean + standard error of the mean (SEM) of 4 independent repeats. Statistically significant differences are indicated with asterisks (**, P < 0.01). (B) RK13 and RN/008 cells were assessed by Western blotting for expression of PRV gB and tubulin (left panel) and by flow cytometry for surface expression of PRV gB (right panel) (black, RK13 cells; dashed line, RN/008 cells; shaded histogram, isotype control).