Experimental scheme for evaluating potential of chemotaxis agents associated with chloroviruses. The microcosms are described in Materials and Methods. (A) In the first experiment, P. bursaria is provided with a choice of cell extracts of mock-infected cells, chlorovirus-infected cells, and pond water. (B) In later experiments, P. bursaria is provided a choice of purified virions suspended in virus stabilization buffer, virus stabilization buffer, or pond water. (C) All experiments were paired with a negative control where all targets were the same (pond water).