FIG 1.

HBV induces HoxA10 expression in cultured cells and patients. (A to D) HoxA10 mRNA and HoxA10 protein expressed in HepG2 and HepG2.2.15 cells (A), HepG2 cells transfected with pBlue-HBV1.3 for 48 h (B), Huh7 cells transfected with pBlue-HBV1.3 for 48 h (C), and HepG2-NTCP cells infected with HBV by inoculation with the supernatants of HepaAD38 cells for 96 h (D) were measured by qPCR (top) and Western blotting (bottom), respectively. (E to H) HBsAg and HBeAg proteins produced in HepG2 and HepG2.2.15 cells (E), HepG2 cells transfected with pBlue-HBV1.3 for 48 h (F), Huh7 cells transfected with pBlue-HBV1.3 for 48 h (G), and HepG2-NTCP cells infected with HBV by inoculation with the supernatants of HepaAD38 cells for 96 h (H) were measured by an ELISA to confirm HBV infection and replication. (I) PBMCs (4 × 10⁶) isolated from healthy individuals were incubated with supernatants of HepG2 cells or supernatants of HepaAD38 cells for 48 h. HoxA10 mRNAs were measured by qPCR. MOI, multiplicity of infection. (J) HoxA10 mRNAs in the leukocytes of CHB patients (n = 32) and healthy individuals (n = 18) were measured by qPCR. (K) Immunohistochemical staining of HoxA10 in paraffin sections of HBV-associated HCC tissues (n = 2) and liver tissues from healthy individuals. (n = 2). *, P < 0.05; **, P < 0.01; ***, P < 0.001.