FIG 2.

HoxA10 represses HBV replication in hepatocytes. (A to F) HepG2 and Huh7 cells were cotransfected with different concentrations of Flag-HoxA10 and pBlue-HBV1.3 for 48 h. (G, H, K, and L) HepG2 and Huh7 cells were cotransfected with different concentrations of si-HoxA10-1 and pBlue-HBV1.3. (I, J, and M) HepG2 and Huh7 cells were cotransfected with sh-HoxA10-1 or sh-HoxA10-2 and pBlue-HBV1.3 for 48 h. (A and G) Total RNAs were extracted from the cells, and HBV RNAs were measured by Northern blot analysis. (B and H) Secreted HBsAg and HBeAg in cell culture supernatants were detected by using an ELISA kit. (C and I) HBV core-associated DNAs were extracted and measured by qPCR. (D and J) HBV cccDNAs were extracted from the cells and measured by qPCR. (E to L) HoxA10 and GAPDH proteins were detected by Western blotting using the corresponding antibodies. (M) The levels of HoxA10 mRNA were measured by RT-PCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001.