FIG 6.

Breast milk and plasma IgAs may inhibit C.1086 HIV-1 virion binding to epithelial cells. Inhibition of binding to the colonic epithelial cell line HT-29 was assessed using the tier 2 clade C virus HIV-1 C.1086, with 7 to 12 replicates performed over three independent experiments. VRC01 IgA was used as a positive control, and HIV-negative colostrum IgA and anti-influenza virus CH65 IgA were used as negative controls. A dotted line indicates the mean percent inhibition (MPI) cutoff of 31%, calculated as 2 standard deviations plus the MPI of anti-influenza virus hemagglutinin MAb CH65 IgA relative to that of the no-antibody condition.