FIG 9.
Functional IgG activity in milk and plasma are correlated and linked to antibodies targeting gp140 and gp120. (A) To determine if the functional activity of anti-HIV-1 antibodies in milk were associated with each other, correlations were tested between neutralization (HIV-1MW965) and phagocytosis (ConS gp140 beads, 1086.C beads, and 92Th023 virions) for milk IgG. Kendall’s tau values and corresponding corrected P values (see Materials and Methods) are reported. Boldface P values indicate significant correlations at a P value of <0.05, with corresponding absolute tau values color coded on a scale of 0 to 1. (B) To determine candidate antigen specificities that could be targeted for functional activity by milk IgG antibodies, correlations were tested between antigen-specific binding and functional activity. (C) To identify whether functional activity was conserved across milk and plasma antibodies, correlations were tested between milk and plasma IgGs for each antibody function (neutralization and phagocytosis).