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Acetylation of K111 by P300 recruits Topo1 to the viral origin. C33A cells were transfected with HPV31 E2 and PFLORI31. This experiment was conducted under endogenous KAT levels and with overexpression of P300 (A) or CBP (B). ChIP for endogenous Topo1 at the HPV31 LCR with anti-Topo1 antibody and LCR3 primers. *, p < 0.05; **, p < 0.005 by one way ANOVA.
