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PV replication is dependent on K111 acetylation by P300. C33A cells were cotransfected with HPV31 E2 K111 mutants, pFLORI31, and pRRL with overexpression of P300 (A) and CBP (B). The luminescence was normalized to renilla luciferase. Each of the samples was compared to their respective control without E1 to cancel out any background transcriptional effects. *, p < 0.05; **, p < 0.005 by paired t test.
