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K111R does not maintain episomes in keratinocytes. Keratinocytes were transfected with HPV31 genomes and selected with puromycin. (A) Cells were maintained for 4 weeks post selection and proliferated at different rates with the E2 mutant genomes. (B) Episomal DNA was isolated, and the presence of HPV31 genomes was detected by PCR. (C) Episomal and genomic DNA isolates were visualized by Southern blotting; (D) differences in the maintenance of genomes and viability of cells were recorded.
