FIG 3.

Luciferase assay to determine the effects of SIRT6 on the promoter activity of ORF50 or ORF59. The ORF50- or ORF59-driven luciferase plasmid was cotransfected with the vector (pcDNA3) or different amounts of SIRT6-expressing plasmid into HEK293T cells for 24 h. The amount of total DNA used in the transfection system was adjusted so that it was the same in each group. Then, the cells were applied to an IVIS imaging system to examine the luciferase activities. (Top) The activities quantitated from three independent experiments were statistically averaged, and the activity units are shown on the y axis. (Bottom) Whole-cell lysate samples were collected after luciferase assay, and Western blotting was performed to detect the expression of SIRT6.