FIG 1.

Illustrations of the replication-incompetent adenovirus vector and KSHV episome copy number analysis using droplet digital PCR (ddPCR). All diagrams are not drawn to scale. (A) Schematic showing the LANA-specific guide RNA and Cas9 expression cassette (CC9-LANA) in the replication-incompetent adenovirus type 5 vector. (B) Relative locations of the ddPCR amplification primers and probes within KSHV LANA and ORF26 genes. (C) The effects of a functional CC9-LANA might have on KSHV episome are presented as three hypothetical scenarios. Predicted ddPCR detectability of the respective probes are shown in tables below each scenario. (D) Example patterns of KSHV episome copy number/cell based on ddPCR analysis that reflects each hypothetical scenarios are shown.