TABLE 2.
Primers and probes for ddPCR
| Primer or probe | Sequence (5′ to 3′)a |
|---|---|
| Forward primer for probes 1 and 2 | AGCCACCGGTAAAGTAGGAC |
| Reverse primer for probes 1 and 2 | GATGTGACCTTGGCGATGAC |
| Probe 1 | FAM-CATGTCGCCGACTCCGTCGA-BHQ1 |
| Probe 2 | HEX-TGCTGGCAGCCCGGATG-BHQ1 |
| Forward primer for probe 3 | GTGTTGTGGCCTAGCTTTCG |
| Reverse primer for probe 3 | CGCGAATACCGCTATGTACTC |
| Probe 3 | HEX- TCTATCTGTCTGTAAGGGACCGGTGG-BHQ1 |
| Forward primer for probe 4 | CGAATCCAACGGATTTGACCTC |
| Reverse primer for probe 4 | CCCATAAATGACACATTGGTGGTA |
| Probe 4 | FAM-CCCATGGTCGTGCCGCAGCA-BHQ1 |
| Forward primer for monkey β-globin probe | CTAGCAACCTCAGACACCATGG |
| Reverse primer for monkey and human β-globin probes | TCCACGTTCACCTTGCCC |
| Monkey β-globin probe | HEX-CTCCTGAGGAGAAGACTGCCGTTACCACC-BHQ1 |
| Forward primer for human β-globin probe | CAACCTCAAACAGACACCATGG |
| Human β-globin probe | HEX-CTCCTGAGGAGAAGTCTGCCGTTACTGCC-BHQ1 |
a
FAM, 6-carboxyfluorescein; BHQ1, black hole quencher 1; HEX, hexagonal peroxisome.