FIG 5.

Accumulation of mRNAs of selected cellular genes in GADD45γ-knockout cells and parental HEp2 cells. HEp-2 or GADD45γ-knockout cells were seeded on 6-well plates, and total RNAs were extracted and reverse transcribed to cDNA, as described in Materials and Methods. mRNAs encoding ATF3, BCL2, HDAC1, HDAC4, IFI16, IFIT1, IRF3, LGP2, LSD1, MDA5, and RIG-I were normalized with respect to 18S RNA. The results shown are the averages and standard deviations for two independent assays, with three replicate samples each. *, P < 0.01, by Student’s t test.