FIG 6.

Accumulation of selected host genes induced by HSV-1(F) infection in GADD45γ-knockout cells. HEp-2 or GADD45γ-knockout cells were seeded on 6-well plates and were infected or mock infected with 5 PFU of HSV-1(F) per cell for 1 h. Cells were then harvested at 2 and 12 h after infection, and total RNAs were extracted and reverse transcribed to cDNA, as described in Materials and Methods. mRNAs encoding IFI16, IFIT1, LGP2, HDAC4, MDA5, and RIG-I were normalized with respect to 18S RNA. The results shown are the averages and standard deviations for two independent assays, with three replicate samples each. *, P < 0.01, by Student’s t test.