FIG 5.

IAV replication and production are enhanced upon inhibiting the de novo pathway. A549 cells were treated with 50 μM FB and/or 0.1 μM Myr or vehicle for 2 h. Cells were then infected with different viral titers of IAV (PR8) at the indicated time points: 1 MOI (a and b) or 0.01 MOI (c and d). Replication of viral RNA was determined by qRT-PCR using gene-specific primers and probes (a and c). Variation in the viral titer in response to treatment was measured by using a plaque assay (b and d). The line represents the lowest detection limit indicated at 2.69 log10 PFU/ml, and ND represents lack of virus detection in the tested samples. Statistical significance was assessed using the t test (*, P < 0.05) relative to vehicle-treated IAV-infected cells (Mock).