TABLE 1.
Primers and probes used in this study
| Target | Primer or probe | Sequence (5′–3′) or sourcea |
|---|---|---|
| M gene | Forward | BEI Resources NR-15594 |
| Reverse | BEI Resources NR-15595 | |
| Probe | BEI Resources NR-15590 | |
| RNase P | Forward | BEI Resources NR-15612 |
| Reverse | BEI Resources NR-15613 | |
| Probe | BEI Resources NR-15611 | |
| vRNA | Reverse transcription | GGCCGTCATGGTGGCGAATGAATGGACGGAGAACAAGGATTC |
| Forward | GGCCGTCATGGTGGCGAAT | |
| Reverse | CTCAATATGAGTGCAGACCGTGC | |
| cRNA | Reverse transcription | GCTAGCTTCAGCTAGGCATCAGTAGAAACAAGGGTATTTTTCTTT |
| Forward | GCTAGCTTCAGCTAGGCATC | |
| Reverse | CGATCGTGCCCTCCTTTG | |
| mRNA | Reverse transcription | CCAGATCGTTCGAGTCGTTTTTTTTTTTTTTTTTCTTTAATTGTC |
| Forward | CCAGATCGTTCGAGTCGT | |
| Reverse | CGATCGTGCCCTCCTTTG | |
| β-Actin | Reverse transcription | Oligo(dT) |
| Forward | ATTGGCAATGAGCGGTTCC | |
| Reverse | GGTAGTTTCGTGGATGCCACA |
a
Tagged sequences are indicated in boldface.