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. Author manuscript; available in PMC: 2020 Mar 21.
Published in final edited form as: Mol Cell. 2019 Feb 12;73(6):1204–1216.e4. doi: 10.1016/j.molcel.2019.01.010

Figure 4.

Figure 4.

PARN, DIS3L2 and DIS3L degrade various miR-21-5p species in vitro with different affinities. In vitro degradation assay for miR-21-5p incubated with immunopurified PARN from A) wild type or B) PARN knockdown cells for indicated time points. FL: Full-length miRNA; Decay: Decay product of the reaction. C), D) and E) In vitro degradation assay for miR-21–5p incubated with immunopurified FLAG-DIS3L2, FLAG-DIS3L and FLAG control respectively for indicated time points. FL: Full-length miRNA; Decay: Decay product of the reaction (Line plot in all cases indicates quantification of average +/− S.D. for three biological replicates). *P<0.05, **P<0.01, two-tailed unpaired Student’s T-test.