Table 2:

Expected TGFβ2 concentration in media from the electrospun scaffolds used in the bioactivity assay and the corresponding expected and obtained cell number. Average % release was obtained from the cumulative release curves presented in Figure 3. Expected TGFβ2 concentration was calculated using the scaffold weight (7 mm piece, 87.5 mg), volume of culture media (150μl), and percentage of release from each scaffold at 5 days. Expected cell number was obtained from the curve presented in Figure 4. The % TGFβ2 release for the 100 ng/mg scaffolds is assumed to be comparable to that from the 13 ng/mg scaffolds.

TGFβ2 Loaded (ng/mg scaffold)	Scaffold	Average % release at 5 days	Expected TGFβ2 concentration (ng/ml)	Expected cell number	Average obtained cell number
	80G:20PCL	1.42	1.07	164.5	259.57
13	50G:50PCL	0.45	0.34	155.8	204.88
	20G:80 PCL	0.038	0.03	145	222.78
	80G:20PCL	1.42	8.28	60	100.92
100	50G:50PCL	0.45	2.63	130	123.62
	20G:80 PCL	0.038	0.22	143.6	229.19