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. Author manuscript; available in PMC: 2019 Jul 28.
Published in final edited form as: Nat Microbiol. 2019 Jan 28;4(4):656–662. doi: 10.1038/s41564-018-0353-x

Figure 1 – Csm6 is required for interference against pG0400 when the target is weakly transcribed.

Figure 1 –

a, The S. epidermidis RP62A type III-A CRISPR, with the catalytic residues of Cas10 and Csm6 highlighted. The black boxes, representing repeats, flank the coloured boxes, symbolising spacers. Spc1 matches the nes antisense transcript of the conjugative plasmid pG0400. b, The architecture around the nes protospacer in pG0400, with RNA-seq traces representing read depth. The annealing positions for spc1 and spc1-flip are indicated. RPM, reads per million. c, Conjugation of pG0400 or pG0420 into S. aureus cells containing pCRISPRs with the indicated csm6 variant and spacer, after filter mating. Each bar represents the mean of three biological replicates ±s.e.m. d, Like b, but for plasmid pG0420, with the inserted transcriptional promoter shown as a black arrow.