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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Leukoc Biol. 2019 Jan 28;105(4):729–740. doi: 10.1002/JLB.3A0818-329RRR

Figure 1. Plasminogen activation in association with cultured BMDMs.

Figure 1.

(A) BMDMs were serum-starved for 30 min and then treated with 12 nM enzymatically-active tPA (EA-tPA), plasminogen (Plg) at the indicated concentrations, and S-2251 (0.2 mM). The plots marked “EA-tPA” and “S-2251” show substrate hydrolysis in cultures treated with EA-tPA and S-2251 in the absence of Plg and with S-2251 alone, respectively. (B) Serum-starved BMDMs were incubated with Plg at the indicated concentrations and S-2251. As a control, S-2251 was added to cultures alone. (C) BMDMs were incubated with EI-tPA, Plg (200 nM or 500 nM), and S-2251. The graphs marked “EI-tPA” and “S-2251” show substrate hydrolysis in cultures treated with EI-tPA and S-2251 in the absence of Plg or S-2251 alone, respectively. The absorbance at 405 nm was determined every 10 min for 70 min. Overlapping data points preclude distinguishing individual points when substrate hydrolysis did not occur (mean ± SEM, n = 6).