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. Author manuscript; available in PMC: 2019 Jul 28.
Published in final edited form as: Nat Microbiol. 2019 Jan 28;4(4):587–594. doi: 10.1038/s41564-018-0345-x

Figure 2: The PGT activity of FtsW is essential for cell division.

Figure 2:

a, In vitro polymerization of Lipid II by co-purified P. aeruginosa FtsW-PBP3 complexes. b, In vitro polymerization of Lipid II by co-purified S. aureus FtsW-PBP1* complexes. Arrowheads (▶) indicate BDL-labeled PBP4 or PBPX formed in the peptidoglycan labeling reaction. c, Phase contrast images of P. aeruginosa cells overexpressing the wild-type FtsW or FtsW*. Scale bar = 10 µm. d, Electron microscopy images of S. aureus cells overexpressing the wild-type FtsW or FtsW*. Scale bar = 500 nm. e, Depletion of FtsW in S. pneumoniae can be rescued by the expression of SpFtsW or StFtsW. Representative data of three (a-b) or two (c-e) independent experiments are shown.