FIGURE 3.

Tam-treated Cx3cr1-Cre^ER;Nhe1^f/f mice decreased pro-inflammatory responses and increased anti-inflammatory responses of microglia/macrophages in the acute stage after ischemic stroke. (a) Representative gating strategies for CD86⁺, Ym1⁺, and CD68⁺ cells within CD11b⁺ microglia/macrophage populations in oil- or Tam-treated Cx3cr1-Cre^ER;Nhe1^f/f brains. (b–f) Cell counts of CD86⁺, CD16/32⁺, Ym1⁺, CD206⁺ and CD68⁺ cells gated under CD11b⁺ cells in oil- and Tam-treated Cx3cr1-Cre^ER;Nhe1^f/f brains at 3 and 7 days Rp, respectively. A total cell count of 50,000 was recorded and analyzed. C, contralateral hemisphere; I, ipsilateral hemisphere. Data are mean ± SEM. At 3 days: N = 5; at 7 days: N = 3. *p < .05, **p < .01. (g) Representative gating strategies for TGF-β⁺, IL-10⁺, and IL-1β⁺ cells within CD11b⁺ microglia/macrophage populations in oil- or Tam-treated Cx3cr1-Cre^ER;Nhe1^f/f brains. (h) Cell counts of TGF-β⁺, IL-10⁺, IL-1β⁺, and TNF-α⁺ cells gated under CD11b⁺ cells in oil- and Tam-treated Cx3cr1-Cre^ER;Nhe1^f/f brains at 3 days Rp, respectively. At least a total cell count of 10,000 was recorded and analyzed. C, contralateral hemisphere; I, ipsilateral hemisphere. Data are mean ± SEM. N = 3. *p < .05, **p < .01, #p < .10 [Color figure can be viewed at wileyonlinelibrary.com]