FIGURE 8.

CamKIIa-Cre^+/−;Nhe1^f/f mice showed delayed activation but no significant effects on polarizations of microglia/macrophages after ischemic stroke. (a) Cell counts of CD11b⁺/CD45^low-med microglia in Ctrl and KO brains before tMCAO and at 3, 7, and 14 days Rp. A total cell count of 50,000 was recorded and analyzed. In all panels C, contralateral; I, ipsilateral. Data are mean ± SEM. N = 3–5. #p < .10 control versus KO groups. (b) Cell counts of CD11b⁺/CD45^hi macrophages in Ctrl and KO brains before tMCAO and at 3, 7, and 14 days Rp. A total cell count of 50,000 was recorded and analyzed. Data are mean ± SEM. N = 3–5. #p < .10 control versus KO groups. (c–g) Cell counts of CD86⁺, CD16/32⁺, Ym1⁺, CD206⁺, and CD68⁺ cells gated under CD11b⁺ cells in Ctrl and KO brains at 3 and 7 days Rp. A total cell count of 50,000 was recorded and analyzed. Data are mean ± SEM. N = 5