Fig. 1.

Synthesis of DSPE-PEG-iRGD and cell-surface modification with DSPE-PEG-iRGD. a Schematic diagram of the synthesis of lipid-conjugated iRGD. b MALDI-TOF characterization of DSPE-PEG-Mal and DSPE-PEG-iRGD construct. The difference in molecular weight indicates the successful connection of iRGD and DSPE-PEG-Mal. c Flow cytometry histograms of T cells alone (grey) and the cells incubated with iRGD-FAM (blue) and DSPE-PEG-iRGD-FAM (red). d Analysis of the percentage of DSPE-PEG-iRGD-FAM modified cell using flow cytometry. e Flow cytometric analysis of changes in relative averaged fluorescence intensities of cells modified with FAM-DSPE-PEG-iRGD over the culture periods. Data represent mean ± s.e.m.; n = 3. T + iRGD, T cells co-administered with free iRGD; T-iRGD, T cells modified with DSPE-PEG-iRGD