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. 2019 Mar 22;10:1352. doi: 10.1038/s41467-019-09333-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Optimization of mouse GIRK2 for native mass spectrometry studies. a Initial preparation of GIRK2 in dodecylmaltoside (DDM) following established protocols for structural studies²⁵. The mass spectrum was recorded using maximum energy settings to reveal 1350 Da adducts bound to GIRK2. b Mass spectrum of GIRK2 in DDM after DHPC treatment, which reveals a well-resolved mass spectrum with the lipid adducts removed. DHPC treated GIRK2 in the charge-reducing detergent, C₁₀E₅ acquired under minimal energy regimes on a c Waters Synapt G1 and d Thermo Exactive plus EMR modified with a rear-entry source⁴⁹. The mass spectra resolves small molecule adducts bound to the tetrameric complex