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. 2019 Mar 22;4:13. doi: 10.1038/s41541-019-0107-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Vaccination with Galα3LN-HSA+/−LMPLA induce T cell activation. Splenocytes were collected from mice immunized with Galα3LN-HSA or Galα3LN-HSA+LMPLA and immunized-challenged mice, followed by ex vivo stimulation with 20 μg/mL Galα3LN-HSA. a, b Percentage of antigen-specific CD4⁺ and CD8⁺ T cells, respectively, in immunized mice. c, d Percentage of CD4⁺ and CD8⁺ T cells, respectively, in immunized-challenged mice. e, f Percentage of memory CD4⁺CD44⁺ and CD8⁺CD44⁺ T cells, respectively, in immunized-challenged mice. One-way analysis of variance (ANOVA) compared with naive group was obtained for the immunized groups; Two-way ANOVA compared with the naive group was obtained for the immunized-challenged groups; *p < 0.05; **p < 0.01; ****p < 0.0001. Error bars indicate S.E.M. of triplicate determinations