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. 2019 Mar 22;9:5029. doi: 10.1038/s41598-019-41326-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Fluorescence emission overlay representing changes due to differences in oxygen concentration 0–14 mg/L of Pt-Porphyrin-PLA nanoparticles loaded hybrid alginate formulation with changing oxygen concentration. (b) Typical dynamic response curve for Pt-Porphyrin loaded PLA nanoparticles when bubbled with Nitrogen and Oxygen. (c,d) Calibration curves for hybrid microparticles in different ranges: 0–13 mM and 0–6 mM. Readings in the calibration curve were determined as mean values of n = 3 experiments and y error bars signify the standard deviation.