Figure 6.

Seahorse metabolic tests of glycolytic and mitochondrial function in D17, Abrams, and Dharma cells. Cells were equilibrated in low‐buffered XF medium for basal readings of oxygen consumption rates (OCR) and extracellular acidification rates (ECAR). (A) Glycolytic test measuring ECAR in each cell line after amiloride (300 μM) or vehicle injection in real time, before the injection of glucose (10 mM), oligomycin (1 μM) and 2‐Deoxy‐D‐glucose (2‐DG) (100 mM). (B) Mitochondrial test measuring OCR in each cell line after amiloride or vehicle injection in real time, before the injection of oligomycin (1 μM), FCCP (0.5 μM), and rotenone/AA (0.5 μM). N = 3 per time point with mean ± SD. AA, antimycin A; FCCP, carbonyl cyanide‐4‐(trifluoromethoxy)phenylhydrazone