Figure 2.

Diagnostic algorithm for immune‐mediated hemolytic anemia (IMHA). Having identified anemia in a patient, biomarkers of immune‐mediated destruction should next be assessed, including the saline agglutination test (SAT), direct antiglobulin test (DAT), and/or flow cytometry (FC); at least 2 should be present, or a positive SAT that persists with washing, to make a firm diagnosis of IMHA. Signs of hemolysis should then be assessed, at least 1 of which should be present for a firm diagnosis. Variations on this theme would yield a supportive or suspicious diagnosis, provided another cause of anemia is not identified. Additional abbreviations: ≥, at least; dz, disease