Fig. 3.

Flow diagram showing steps taken during OZ439 in vitro solubilization tests. Following intestinal fluid addition in step 3, the maximum theoretical concentration of OZ439 was approximately 0.535 mg/mL. Pelleted NPs (after step 5) or bile salts (after step 7) are denoted by white ovals. As dissolution matching 100% of theoretical dissolution was achieved via this protocol, we found that the method results in negligible OZ439 losses despite its several steps