Fig. 5.

ID4 was a direct target of miR-342-3p in PC. a Schematic representation of binding sites between miR-342-3p and ID4 predicted by TargetScan software. b Luciferase activity assay was performed after co-transfection with reporter plasmid and miRNAs into HEK293T cells. c, d The protein levels of ID4 in PANC-1 and PaCa-2 cell lines were determined by western blot with transfection of miR-342-3p or negative control. e The expression mRNA levels of ID4 in PC tissue sand adjacent normal ones were determined by RT-qPCR. f Negative correlation between miR-342-3p and ID4 in PC tissues. **P < 0.01, compared with miR-NC group