Fig. 6.

ITSA-1 reverses TSA-impacted changes in NPC cells. a CCK-8 assay was used to detect the cell viability of TSA (200 ng/ml) and TSA + ITSA-1(10 μmol/l) - treated CNE2 and C666–1 cells. N.S., no significance; ***P < 0.001. b Western blot was used to examine the protein levels of cell proliferation (PCNA), cell cycle (cyclinD1, CDK2 and p16) and EMT-associated genes (E-cadherin and Vimentin). c Morphology of TSA and ITSA-impacted NPC cells. Scale bars = 50 μm. d & e Scratch wound healing assay was performed to test the migration ability of CNE2 and C666–1 cell treated with TSA or TSA + ITSA-1 for 48 h. Scale bars = 200 μm. *P < 0.05; **P < 0.01