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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Leukoc Biol. 2018 Jul 5;104(4):677–689. doi: 10.1002/JLB.4HI0817-333RR

Figure 9. dnHMGA1 cells and mice have decreased expression of chemokines during exposure to imflammatory stimuli.

Figure 9.

SMCs harvested from WT or dnHMGA1 (Tg1) mice were exposed to vehicle (Veh), IL-1β (10 ng/ml), or TNF-α (10 ng/ml) for various time points (as depicted). Total RNA was extracted from the cells, and levels of (A) MCP-1 and (B) MIP-2 were measured by qRT-PCR. Data are presented as mean ± SEM, n=3 per group, with testing by one-way ANOVA (P<0.0001). Significant comparisons; * WT versus Tg1. C) WT and Tg1 mice underwent sham (– E. coli) or fibrin clot (+ E. coli) surgery. At 24 hours after surgery, tissue (spleen) and plasma were collected, and the concentration of MCP-1 was assessed using a Luminex bead assay as described in the Material and Methods. Data are represented as mean±SEM, from 3 mice per group. Analyses by one-way ANOVA (P=0.0399, spleen; P=0.0253, plasma). Significant comparisons for A and B, * versus sham groups, † versus WT E. coli group. NS = not significant.