(A) Schematic representation of DMPC membrane and a thermoresponsive PNIPAM particle as a function of temperature. Below the melting temperature, , the DMPC forms gel-phase bilayers with solid chains, and above , DMPC forms bilayers with fluid chains. In the investigated temperature range, the microgel particles are swollen and hydrophilic. (B–G) Fluorescence confocal micrographs showing DMPC GUVs decorated with PNIPAM particles at 30 °C (B and E), 24 °C (C and F), and 17 °C (D and G). B–D, Insets reveal close-up views of lateral cross-sections of GUV-microgels contact lines. (E–G) The 3D intensity projections of confocal -stacks, supported by close-up views of particle organizations at the surface of the GUVs shown as Insets. (Scale bars: 5 m.)