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. 2019 Feb 28;116(12):5570–5575. doi: 10.1073/pnas.1814930116

Fig. 3.

Fig. 3.

Gonad innervation is neural crest-derived. (A and B) Lineage-trace of neural crest-derived neurons in whole urogenital complexes from XY (A) and XX (B) Wnt1Cre; Rosa-tdTomato embryos at E15.5. (C and D) Lineage-trace of neural crest-derived progenitors in gonads from XY (C) and XX (D) Wnt1Cre; Rosa-tdTomato neonates (P0). C′ and D′ are magnified views in single channels of the areas outlined in C and D. Samples were stained for red fluorescent protein (RFP) to detect tdTm expression (red, A and D), the pan-neuronal marker TUJ1 (green, A and B), and the pan-neuronal marker DCX (green, C and D). XY samples were stained for the Sertoli cell marker AMH to label the testis (cyan, A′), and XX samples were stained for the granulosa cell marker FOXL2 to label the ovary (cyan, B′). All samples were counterstained with Hoechst nuclear dye (grayscale). (Scale bars, 100 µm.)