T-CTLs were enriched from other subsets by cell sorting for either NKG2A (A+) or NKG2C (C+). (A) Each population was stimulated with anti-CD94 or left in medium for 20 hours. GZMB, PRF, and GNLY release was measured in the culture supernatants by ELISA. The log % change of the anti-CD94 stimulation relative to medium is shown. (B) Each population was stimulated with anti-CD3 or anti-CD3 and anti-CD94 for 20 hours, and cytotoxic granule proteins were measured by ELISA. The log % change of the anti-CD94 plus anti-CD3 relative to anti-CD3 alone is shown. *P < 0.05, **P < 0.01, ***P < 0.001.