Table 1.
Gene | Sequence | AMPL. size | NCBI ref. seq. | |
---|---|---|---|---|
GAPDH | Fw: | 5′- GGAGCGAGATCCCTCCAAAAT -3′ | 197 bp | NM_001256799 |
Rev: | 5′- GGCTGTTGTCATACTTCTCATGG -3′ | |||
VEGF | Fw: | 5′- AGGGCAGAATCATCACGAAGT -3′ | 75 bp | NM_001171627 |
Rev: | 5′- AGGGTCTCGATTGGATGGCA -3′ | |||
Bcl-2 | Fw: | 5′- GGTGGGGTCATGTGTGTGG-3′ | 89 bp | NM_000657 |
Rev: | 5′- CGGTTCAGGTACTCAGTCATCC-3′ | |||
BAX | Fw: | 5′- CCCGAGAGGTCTTTTTCCGAG-3′ | 155 bp | NM_138763 |
Rev: | 5′- CCAGCCCATGATGGTTCTGAT-3′ | |||
LC3B | Fw: | 5′- GATGTCCGACTTATTCGAGAGC-3′ | 167 bp | NM_022818 |
Rev: | 5′- TTGAGCTGTAAGCGCCTTCTA-3′ | |||
Caspase 3 | Fw: | 5′- CATGGAAGCGAATCAATGGACT-3′ | 139 bp | NM_004346 |
Rev: | 5′- CTGTACCAGACCGAGATGTCA-3′ | |||
mTOR | Fw: | 5′- ATGCTTGGAACCGGACCTG-3′ | 173 bp | NM_004958 |
Rev: | 5′- TCTTGACTCATCTCTCGGAGTT-3′ | |||
Beclin-1 | Fw: | 5′- CCATGCAGGTGAGCTTCGT-3′ | 215 bp | NM_003766 |
Rev: | 5′- GAATCTGCGAGAGACACCATC-3′ |
Fw = forward; Rev = reverse.