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. 2019 Mar 6;2019:2039856. doi: 10.1155/2019/2039856

Figure 6.

Figure 6

Effects of Nrf2 overexpression on mechanical trauma-induced alterations of oxidative damage and antioxidative capacity. (a–c) Immunohistochemistry was used to detect the levels of 8-OHdG and 4-HNE in the anterior wall of mice in three groups. Brown represents the positive signal. Scale bars: 100 μm. (d) The MDA measurement kit was used to detect the levels of MDA in the anterior wall of mice in three groups. (e–g) CAT, GSH-PX, and T-SOD measurement kits were used to detect the activities of CAT, GSH-PX, and T-SOD, respectively, in the anterior wall of mice in three groups. ∗ P < 0.05; NS: no significant difference. Every experiment was repeated 3 times and representative figures are presented here. 8-OHdG: 8-oxo-2′-deoxyguanosine; 4-HNE: 4-hydroxynonenal; MDA: malondialdehyde; CAT: catalase; GSH-PX: glutathione peroxidase; T-SOD: total superoxide dismutase; VD: vaginal distension; WT-VD7d: mice in control group and evaluated on day 7 after VD; Vector-VD7d: mice transfected with LV vector and evaluated on day 7 after VD; Nrf2-OV: mice transfected with LV-Nfe2l2 for in vivo Nrf2 overexpression and evaluated on day 7 after VD.